Cholesterol (tot.)

Analyt: Cholesterol (tot.)
Matrix: Serum
Measuring Method: GC-ID/MS
Standard: Cholesterol NIST 911c
Internal Standard: ¹³C₂ Cholesterol
Sample Processing: Samples are spiked gravimetrically with internal standard and equilibrated, then hydrolysed with fresh methanolic KOH and extracted with n-Hexane. Derivatisation is performed with MSTFA.
Measurement: Measurement at m/z 458 and m/z 460