Cholesterol (tot.)
Analyt: | Cholesterol (tot.) |
Matrix: | Serum |
Measuring Method: | GC-ID/MS |
Standard: | Cholesterol NIST 911c |
Internal Standard: | ¹³C₂ Cholesterol |
Sample Processing: | Samples are spiked gravimetrically with internal standard and equilibrated, then hydrolysed with fresh methanolic KOH and extracted with n-Hexane. Derivatisation is performed with MSTFA. |
Measurement: | Measurement at m/z 458 and m/z 460 |