Glycerol (tot.)

Analyt: Glycerol (tot.)
Matrix: Serum
Measuring Method: GC-ID/MS
Standard: Tripalmitin NIST 1595
Internal Standard: ¹³C₃-Triolein
Sample Processing: Samples are spiked gravimetrically with internal standard and equilibrated, then hydrolysed with fresh methanolic KOH and neutralised with HCl followed by extraction into acetone. Derivatisation with HFBA and extraction into n-hexane.
Measurement: Measurement at m/z 467 and m/z 470