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Progesterone

Analyt: Progesterone
Matrix: Serum
Measuring Method: GC-ID/MS
Standard: Progesterone NMIJ CRM 6003-a
Internal Standard: ¹³C₂-Progesterone
Sample Processing: Samples are spiked gravimetrically with internal standard and equilibrated, then extracted into n-Hexane, followed by centrifugation and evaporation of the supernatant to dryness. Derivatisation is with HFBA in cyclohexane.
Measurement: Measurement at m/z 510 and m/z 512