Cholesterol (tot.)
| Analyt: | Cholesterol (tot.) |
| Matrix: | Serum |
| Measuring Method: | GC-ID/MS |
| Standard: | Cholesterol NIST 911c |
| Internal Standard: | ¹³C₂ Cholesterol |
| Sample Processing: | Samples are spiked gravimetrically with internal standard and equilibrated, then hydrolysed with fresh methanolic KOH and extracted with n-Hexane. Derivatisation is performed with MSTFA. |
| Measurement: | Measurement at m/z 458 and m/z 460 |