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17ß-Estradiol

Analyt: 17ß-Estradiol
Matrix: Serum
Measuring Method: GC-ID/MS
Standard: 17ß-Estradiol NMIJ CRM 6004-a
Internal Standard: ¹³C₂-Estradiol
Sample Processing: Samples are spiked gravimetrically with internal standard, equilibrated, then extracted into Dichloromethane, followed by a cleaning up step with Sephadex LH-20. Derivatisation is with cyclohexane/acetone/HFBA
Measurement: Measurement at m/z 664 and m/z 666