17ß-Estradiol
| Analyt: | 17ß-Estradiol |
| Matrix: | Serum |
| Measuring Method: | GC-ID/MS |
| Standard: | 17ß-Estradiol NMIJ CRM 6004-a |
| Internal Standard: | ¹³C₂-Estradiol |
| Sample Processing: | Samples are spiked gravimetrically with internal standard, equilibrated, then extracted into Dichloromethane, followed by a cleaning up step with Sephadex LH-20. Derivatisation is with cyclohexane/acetone/HFBA |
| Measurement: | Measurement at m/z 664 and m/z 666 |