17ß-Estradiol
Analyt: | 17ß-Estradiol |
Matrix: | Serum |
Measuring Method: | GC-ID/MS |
Standard: | 17ß-Estradiol NMIJ CRM 6004-a |
Internal Standard: | ¹³C₂-Estradiol |
Sample Processing: | The samples are gravimetrically spiked with internal standard, equilibrated, extracted with dichloromethane and further purified with Sephadex LH-20. Derivatization is with tetrahydrofuran/HFBA. |
Measurement: | Measurement at m/z 664 and m/z 666 |