Analyt: Digitoxin
Matrix: Serum
Measuring Method: LC-ID/MS
Standard: LGC Reference Material / Product Code DER-C12633800
Internal Standard: Digitoxin-d₃
Sample Processing: Samples are spiked gravimetrically with internal standard, equilibrated, then extracted with a liquid-liquid phase (TBME/Ethyl acetate) before being evaporated and taken up into HPLC-buffer
Measurement: Measurement at m/z 897 and m/z 900 (Cs adducts) or at MS/MS with m/z 132.9 amu (Cs ion)