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Digoxin

Analyt: Digoxin
Matrix: Serum
Measuring Method: LC-ID/MS
Standard: Digoxin ERM-AC200a (LGC)
Internal Standard: Digoxin-d₃
Sample Processing: Samples are spiked gravimetrically with internal standard, equilibrated, then extracted with a liquid-liquid phase (TBME/Ethyl acetate) before being evaporated and taken up into HPLC-buffer
Measurement: Measurement at m/z 913 and m/z 916 (Cs adducts) or at MS/MS with m/z 132.9 amu (Cs-ion)