Digoxin
Analyt: | Digoxin |
Matrix: | Serum |
Measuring Method: | LC-ID/MS |
Standard: | Digoxin ERM-AC200a (LGC) |
Internal Standard: | Digoxin-d₃ |
Sample Processing: | Samples are spiked gravimetrically with internal standard, equilibrated, then extracted with a liquid-liquid phase (TBME/Ethyl acetate) before being evaporated and taken up into HPLC-buffer |
Measurement: | Measurement at m/z 913 and m/z 916 (Cs adducts) or at MS/MS with m/z 132.9 amu (Cs-ion) |