Analyt: Glucose
Matrix: Serum, Urine, CSF
Measuring Method: GC-ID/MS
Standard: D-Glucose NIST 917c
Internal Standard: ¹³C₆-Glucose
Sample Processing: Samples are spiked gravimetrically with internal standard and equilibrated, then treated with methanol to precipitate proteins. Derivatisation is with hydroxyl-ammonium chloride in pyridine and acetic anhydride with subsequent extraction into dichloromethane.
Measurement: Measurement at m/z 314 and m/z 319