Testosterone
| Analyt: | Testosterone |
| Matrix: | Serum |
| Measuring Method: | GC-ID/MS |
| Standard: | Testosterone NMIJ CRM 6002-a |
| Internal Standard: | ¹³C₂-Testosterone |
| Sample Processing: | Samples are spiked gravimetrically with internal standard and equilibrated, then precipitated with aqueous KOH, centrifuged and the supernatant extracted into dichloromethane. Derivatisation is with cyclohexane-HFBA and subsequent extraction into cyclohexane. |
| Measurement: | Measurement at m/z 680 and m/z 682 |